Activation of Gαi and subsequent uncoupling of receptor-Gαi signaling by Pasteurella multocida toxin
نویسندگان
چکیده
Joachim H. C. Orth, Ines Fester, Inga Preuß, Laura Agnoletto, Brenda A. Wilson and Klaus Aktories Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Albert-LudwigsUniversität Freiburg, D-79104 Freiburg, Germany, Department of Microbiology, University of Illinois at Urbana-Champaign, 601 South Goodwin Avenue, B128 CLSL, Urbana, IL 61801, USA. Running title: Pasteurella multocida toxin activates Gαi Address correspondence to: Dr. Klaus Aktories, Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Albertstraße 25, D-79104 Freiburg, Germany, Tel. +49-7612035301, Fax +49-761-2035311, E-mail: [email protected]
منابع مشابه
The actions of Pasteurella multocida toxin on neuronal cells☆
Pasteurella multocida toxin (PMT) activates the G-proteins Gαi(₁₋₃), Gα(q), Gα₁₁, Gα₁₂ and Gα₁₃ by deamidation of specific glutamine residues. A number of these alpha subunits have signalling roles in neurones. Hence we studied the action of this toxin on rat superior cervical ganglion (SCG) neurones and NG108-15 neuronal cells. Both Gα(q) and Gα₁₁ could be identified in SCGs with immunocytoche...
متن کاملPasteurella multocida Toxin Manipulates T Cell Differentiation
Pasteurella multocida causes various diseases in a broad range of wild and domestic animals. Toxigenic strains of the serotypes A and D produce an AB protein toxin named Pasteurella multocida toxin (PMT). PMT constitutively activates the heterotrimeric G protein subunits Gαq, Gα13, and Gαi through deamidation of a glutamine residue, which results in cytoskeletal rearrangements as well as increa...
متن کاملPasteurella multocida toxin as a transporter of non-cell-permeating proteins.
The protein toxin Pasteurella multocida toxin (PMT) is the causative agent of atrophic rhinitis in pigs, leading to atrophy of the nasal turbinate bones by affecting osteoblasts and osteoclasts. The mechanism of PMT-induced intoxication is a deamidation of α-subunits of heterotrimeric G proteins, including Gαq, Gα13, and Gαi, thereby causing persistent activation of the G proteins. Here we util...
متن کاملA New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells
G-protein coupled receptors (GPCRs) can activate a heterotrimeric G-protein complex with subsecond kinetics. Genetically encoded biosensors based on Förster resonance energy transfer (FRET) are ideally suited for the study of such fast signaling events in single living cells. Here we report on the construction and characterization of three FRET biosensors for the measurement of Gαi1, Gαi2 and G...
متن کاملDirect Coupling of a Seven-Transmembrane-Span Receptor to a Gαi G-Protein Regulatory Motif Complex.
Group II activator of G-protein signaling (AGS) proteins contain one or more G-protein regulatory motifs (GPR), which serve as docking sites for GαiGDP independent of Gβγ and stabilize the GDP-bound conformation of Gαi, acting as guanine nucleotide dissociation inhibitors. The GαGPR interaction is regulated by seven-transmembrane-spanning (7TM) receptors in the intact cell as determined by biol...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008